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Nevertheless, through the time system for measurement of catalytic activity via the apo-enzyme, the speed of substrate turnover enhanced slowly but surely with time. The activation from the apo-enzyme was seemingly a result of the binding of trace metals in the assay Remedy since the activity on the apo-enzyme while in the presence of 500 μM EDTA was lower than 2% from the exercise exhibited with the native enzyme. These results are in keeping with an absolute prerequisite for just a certain divalent cation with the expression of catalytic activity by NagA.
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m whilst the Zn enzyme has the bottom. Identical tendencies within the choice of the catalytic constants were noted Beforehand for the Co, Zn, Mn and Fe reconstitutions of apo-NagA from E. coli
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The slot naga26 effects of solvent viscosity over the catalytic price constants for that hydrolysis of one by Zn-NagA are proven in Figures 4A and 4B applying sucrose given that the micro-viscogen. The plot daftar naga26 of relative k
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